Studi In silico Potensi aktivitas 10-shogaol dalam Jahe (Zingiber officinale) terhadap gen ACE

Mansur S, Yohanes Bare, Maria Helvina, Aprianus Pani Pili, Gabriella Chandrakirana Krisnamurti

Abstract


Peningkatan tekanan darah tinggi (hipertensi) mengakibatkan terpicunya berbagai macam penyakit kronis serta komplikasinya. Salah satu activator dalam kondisi tekanan darah tinggi adalah aktivitas Angiotensin converting enzyme (ACE). ACE berperan dalam mengatur sistem renin-angiotensin. Penurunan hipertensi perlu untuk menekan aktivitas ACE. Tujuan dari penelitian ini adalah untuk menganalisis potensi 10-shogaol terhadap ACE. Metode yang digunakan adalah studi in silico. Protein model ACE (ID: 3bkk) didapatkan dari database Protein data Bank (PDB) sedangkan 10-shogaol (CID: 6442612) didapat dari database PubChem. Docking menggunakan Hex 8.0.0 sedangkan visualisasi dan analisis menggunakan Discovery Studio. Hasil Penelitian menunjukan bahwa bahwa senyawa 10-shogaol memiliki potensi sebagai inhibitor gen ACE dibuktikan 2 residu asam amino yang berikatan dengan ligan. Ikatan tersebut berupa ikatan hidropobik yang meningkatkan kekuatan tersebut dengan besar energi -291.3cal/mol.

Keywords


10-shogaol; ace; in silico; inhibitor; jahe

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References


S. Singh, R. Shankar, and G. P. Singh, “Prevalence and Associated Risk Factors of Hypertension: A Cross-Sectional Study in Urban Varanasi,” Int. J. Hypertens., vol. 2017, pp. 1–10, 2017, doi: 10.1155/2017/5491838.

World Helath Organization, Global status report on noncommunicable diseases 2010. Italy: World Helath Organization, 2011.

Y. Bare, A. Marhendra, T. Sasase, and F. Fatchiyah, “Differential Expression of IL-10 Gene and Protein in Target Tissues of Rattus Norvegicus Strain Wistar Model Type 2 Diabetes Mellitus (T2DM),” Acta Inform. Medica, vol. 26, no. 2, pp. 87–92, 2018, doi: 10.5455/aim.2018.26.87-92.

W. Ouwerkerk et al., “Determinants and clinical outcome of uptitration of ACE-inhibitors and beta-blockers in patients with heart failure: a prospective European study,” Eur. Heart J., vol. 38, no. 24, pp. 1883–1890, Jun. 2017, doi: 10.1093/eurheartj/ehx026.

Q.-Q. Mao et al., “Bioactive Compounds and Bioactivities of Ginger (Zingiber officinale Roscoe),” Foods, vol. 8, no. 6, p. 185, May 2019, doi: 10.3390/foods8060185.

A. Ghasemzadeh, H. Jaafar, A. Baghdadi, and A. Tayebi-Meigooni, “Formation of 6-, 8- and 10-Shogaol in Ginger through Application of Different Drying Methods: Altered Antioxidant and Antimicrobial Activity,” Molecules, vol. 23, no. 7, p. 1646, Jul. 2018, doi: 10.3390/molecules23071646.

H. N. Meidinna and Fatchiyah, “The Potential Role of Rosmarinic Acid and Sinensetin as α- Amylase Inhibitor: In Silico Study,” J. Pure Appl. Chem. Res., vol. 8, no. 1, pp. 73–79, Apr. 2019, doi: 10.21776/ub.jpacr.2019.008.001.460.

Y. Bare, M. S, S. H. J. Putra, M. R. W. G. L, and D. R. T. Sari, “In-silico Approach for The Prediction of Chlorogenic Acid as PPAR-γ Activator,” Biota, vol. 13, no. 1, Jun. 2020, doi: 10.20414/jb.v13i1.197.

D. Sari, J. Cairns, A. Safitri, and F. Fatchiyah, “Virtual Prediction of the Delphinidin-3-O-glucoside and Peonidin-3-O-glucoside as Anti-inflammatory of TNF- and #945; Signaling,” Acta Inform. Medica, vol. 27, no. 3, p. 152, 2019, doi: 10.5455/aim.2019.27.152-157.

S. S. N. D. Tiring, Y. Bare, A. Maulidi, M. S, and F. A. D. Nugraha, “Studi In Silico: Prediksi Potensi 6-shogaol dalam Zingiber officinale sebagai Inhibitor JNK,” Al-Kim., vol. 7, no. 2, pp. 147–153, 2019, doi: 10.24252/al-kimia.v7i2.10638.

Y. Bare, A. D. Kuki, A. H. Rophi, and G. Candrakirana, “Prediksi Asam Kuinat Sebagai Anti-Inflamasi Terhadap COX-2 Secara Virtual Virtual Prediction of Quinic Acid As Anti-Inflammation of COX-2,” Biota J. Ilmu-Ilmu Hayati, vol. 4, no. 3, pp. 124–129, 2019, doi: https://doi.org/10.24002/biota.v4i3.2516.

Y. Bare, D. R. T. Sari, Y. T. Rachmad, G. C. Krisnamurti, and A. Elizabeth, “In Silico Insight the Prediction of Chlorogenic Acid in Coffee through Cyclooxygenase-2 (COX2) Interaction,” Biog. J. Ilm. Biol., vol. 7, no. 2, pp. 100–105, Dec. 2019, doi: 10.24252/bio.v7i2.9847.

K. H. Choi, L. Kremer, G. S. Besra, and C. O. Rock, “Identification and substrate specificity of β-Ketoacyl (Acyl carrier protein) synthase III (mtFabH) from mycobacterium tuberculosis,” J. Biol. Chem., vol. 275, no. 36, pp. 28201–28207, 2000, doi: 10.1074/jbc.M003241200.

B. Jia, Y. M. Ma, B. Liu, P. Chen, Y. Hu, and R. Zhang, “Synthesis, Antimicrobial Activity, Structure-Activity Relationship, and Molecular Docking Studies of Indole Diketopiperazine Alkaloids,” Front. Chem., vol. 7, no. November, pp. 1–13, 2019, doi: 10.3389/fchem.2019.00837.

D. R. T. Sari and Y. Bare, “Physicochemical properties and biological activity of bioactive compound in Pepper nigrum: In silico study,” vol. 1, no. 2, p. 6, 2020.

K. Erdmann, B. W. Y. Cheung, and H. Schröder, “The possible roles of food-derived bioactive peptides in reducing the risk of cardiovascular disease,” J. Nutr. Biochem., vol. 19, no. 10, pp. 643–654, Oct. 2008, doi: 10.1016/j.jnutbio.2007.11.010.

L. Actis-Goretta, J. I. Ottaviani, and C. G. Fraga, “Inhibition of Angiotensin Converting Enzyme Activity by Flavanol-Rich Foods,” J. Agric. Food Chem., vol. 54, no. 1, pp. 229–234, Jan. 2006, doi: 10.1021/jf052263o.

Bare, Y., Maulidi, A., Sari, D. R. T., & Tiring, S. S. N. D. (2019). Studi in Silico Prediksi Potensi 6-Gingerol sebagai inhibitor c-Jun N-terminal kinases (JNK). Jurnal Jejaring Matematika dan Sains, 1(2), 59–63. https://doi.org/10.36873/jjms.v1i2.211

Bare, Y., Sari, D. R., Rachmad, Y. T., Tiring, S. S. N. D., Rophi, A. H., & Nugraha, F. A. D. (2019). Prediction Potential Chlorogenic Acid As Inhibitor Ace (In Silico Study). Bioscience, 3(2), 197. https://doi.org/10.24036/0201932105856-0-00

Bare, Y., S, M., Tiring, S. S. N. D., Sari, D. R. T., & Maulidi, A. (2020). Virtual Screening: Prediksi potensi 8-shogaol terhadap c-Jun N-Terminal Kinase (JNK). Jurnal Penelitian Dan Pengkajian Ilmu Pendidikan: E-Saintika, 4(1), 1–6. https://doi.org/10.36312/e-saintika.v4i1.157




DOI: http://dx.doi.org/10.55241/spibio.v1i3.23

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